(Focht Live-Cell Chamber System) Instructions
A Message from the Developer:
FCS2 utilizes the most advanced technology for optimum functionality and
versatility for the observation of living cells on a light microscope.
As part of our dedication to respond to our customers needs we
would be very appreciative of any comments or suggestions you may have
relative to our products continued improvement.
Before You Begin:
Check to make sure there is no
visible damage in shipment and save all packing materials.
The Controller box should
contain; controller, power cord, 2.5mm screwdriver, 1/8” jack, and 3
pin mini DIN connector.
· Check the packing list and call your supplier if there are any discrepancies.
Front Panel of FCS2 & FCS3 Controller
Display should be illuminated when
the controller is turned on. If it does not light, check the main power source, then the
fuse block located in the power input module where the AC power cord
Selector is the black button
adjacent to the display. It
selects the source of the information displayed.
When the controller is turned on, the default condition is to
display the setpoint value indicated by the red light adjacent to the
The button selects one of a sequence of display options.
This button can be pressed at any time and has no effect on the
temperature regulation functions of the controller.
Lights indicate the source of the
value displayed on the numeric display.
is adjusted by a potentiometer accessible with the 2.5mm screwdriver
provided. To adjust the
setpoint set the numeric display to Setpoint,
then adjust the potentiometer to the desired value.
If you need to change the setpoint value during your experiment
on a routine basis there is a remote setpoint jack on the back of the
controller. See Remote
is the location where the
calibration of the circuit reading the Microaqueduct Slide can be
is the location where the
calibration of the circuit reading the Chamber can be adjusted.
(Red Button) resets the controller if the alarm sounds.
Note: If the alarm
went off there must have been a reason.
Check the chamber before resetting the controller.
substitutes precision resistors into the circuit that reads the
thermistors so that calibration adjustments can be made to a known
a six pin mini DIN connector where the FCS2 or FCS3 is plugged in.
Panel of the FCS2 & FCS3 Controller
line input, power switch, voltage selector, and fuses are all
incorporated in the AC Power Input Module.
The controller can operate between 90 - 260V 50-60Hz, or a 24V
battery can be used as an alternate power supply.
The fuse carrier contains two 0.5A slow blow fuses.
The fuses can be accessed by prying the fuse block out of its
socket with a small screwdriver wedged under the small slot in the
opening of the AC line receptacle.
When replacing the fuse block, make sure to align the arrow on
the outside of the fuse block with the closest voltage value to your
supply line voltage:
For 90-130 supply line voltage
place the fuse block with the arrow pointing to 110-120V.
For 180-260 supply line voltage
place the fuse block with the arrow pointing to 220-240V.
Out is a voltage output direct from
two instrument amplifiers reading the thermistors from channel A and B.
The output from this
socket is temperature / 10. 37.0°C
= 3.70 Volt DC.
The left pin is channel
A, center pin is channel B, and right pin is ground.
Setpoint is an analog input that
allows the user to set the controllers setpoint from an external DC
source. The sleeve is earth ground. A DC voltage can be applied to the tip of this jack equal to
temperature /10. i.e.: 28.5°C
= 2.85 Volt. When the jack
is inserted into the controller it will automatically switch to the
jacks value. When removed
it will default to the setting on the front of the controller.
Use for programming or cycling operating temperatures.
is a safety cut-off circuit that protects the cells if the microaqueduct
drops below 0.9°C from the setpoint after reaching setpoint minus 0.9°C.
Only during a cold start will the safety cut-off circuit ignore
the out-of-range condition. This safety circuit will shut off power to the slide
circuit only and sound the alarm. If
the alarm sounds, the user should correct the problem and press the red
reset button located next to the red LED.
alarm may sound as a result of the following conditions:
Perfusion rate exceeds the rate
which temperature stability can be maintained. To correct, press the red reset button as soon as the alarm
sounds. This will reset the
logic back to cold start up mode. Setpoint temperature will return
within 15-20 seconds.
An interruption in AC line power
may cause the logic levels to change state.
Physical displacement of the
thermistor from the microaqueduct surface.
The thermistor that regulates the chamber frame is sealed
internally eliminating accidental displacement.
Therefore, there is no need for an alarm on the chamber frame
chamber has been inspected prior to packaging and is ready to use when
you receive it. The chamber
is shipped assembled and should be opened using the following procedure.
Rotate the large knurled ring in the
base of the chamber clockwise until it stops.
This will release and disengage the chamber top.
Hold the white portion of the chamber while simultaneously (using
thumb and forefinger) rotating CCW the two smaller knurled nuts located
on either side of the black electrical contact unit.
This will remove the black portion which contains sensitive
electronics so that they can remain dry.
You now have the basic components of the chamber separated. Continue with the following directions:
Directions for Closed System Use: (see
drawing for part identification)
Attach perfusion tubing to the
inlet and outlet ports of the chamber.
Bioptechs recommends 1/16" Tygon #2275 tubing for best
biocompatibility and ease of use. See
Turn the top of the chamber
(white) upside-down. Place
the upper gasket (.75mm thick) into the recess while aligning the
perfusion clearance holes with the perfusion tubes.
Place the microaqueduct slide on
the upper gasket so that it is aligned with perfusion tubes (grooved
Place the lower gasket (0.1mm -
1.0mm thick) onto the microaqueduct slide and press around the perimeter
creating a seal.
Perfuse some media through the
perfusion port producing a bead on the surface of the slide to displace
air trapped in the line.
Lower the coverslip with cells
onto the bead until it is resting on the gasket.
Place the closure assembly on
top of this stack while aligning the black electrical connector with the
oval slot in the chamber top. Maintain
a gentle pressure while turning the chamber over so that it is right
side up then can observe as the four paws engage the top through the
depressions about the perimeter. Turn
the large knurled ring counter-clockwise until tight.
This will symmetrically tighten and seal the chamber.
For applications involving rapid
perfusion, a drop of immersion oil can be placed at the point where the
base of the chamber meets the coverslip in the exposed aperture of the
chamber. This drop will be
drawn in by capillary action and enhance thermal conductivity to the
coverslip. Place a light
film of immersion oil on the flat surface of the surface probe to
enhance thermal conductivity.
Place the black power/sensor
connector back on the two threaded posts and symmetrically rotate CW
(using thumb and forefinger) the two knurled nuts onto the post.
Make sure the spring wires make contact with the bus bars on the
surface of the microaqueduct slide.
It is important that the flat
side of the surface probe is making uniform contact with the
microaqueduct slide because the feedback loop that controls the
microaqueduct temperature is reliant on this thermal contact point.
Plug the FCS2 Chamber into the
controller (6 Pin mini DIN).
The display can be switched
between Set, Slide and Chamber at any time to monitor temperature
without interfering with the regulation.
If the Chamber was preheated it will come up to temperature in
about 1 minute. Otherwise it will take longer.
The Chamber should now be
mounted onto the microscope adapter in the stage.
The vertical portion of the
drain tubing which extends down from the stage to your waste receptacle
will create a siphon and form negative pressure in the chamber.
This negative pressure will cause the coverslip to flex.
This can be eliminated by breaking the siphon at a point equal to
the height of the specimen with a "T" fitting.
various components of the chamber can be cleaned as follows:
High Numeric Aperture Use:
you are using high numeric aperture (immersion) objectives with high N.A.
dishes, it will be necessary to regulate the temperature of the
objective as well. This is
due to the fact that the optical coupling medium, oil, glycerin or water
also has a thermal coupling effect.
In this case, a Bioptechs, Inc. Objective Heater and an Objective
Heater Controller will be necessary for uniform temperature across the
Objective Heater Description:
Bioptechs Objective Heater is an adjustable, thin film, size and shape
compliant, heating band and temperature sensor assembly attached to a
linear translator. This
device attaches to the objective and plugs into a separate Temperature
Controller, which regulates the objective temperature within 0.1°C of
Continual Perfusion with the Micro-Perfusion
Bioptechs recommends the FCS2 Micro-Perfusion Pump due to its smooth flow profile in slow, flow applications. If dual flow is needed there is also a PC interface board and program that enables alternate control of two pumps to regulate the acceleration and run time of each. The Micro-Perfusion Pump Controller (PC interface) is ideal for changing concentrations of media.