Objective Heater System
Before You Begin:
Check to make sure there is no
visible damage in shipment and save all packing materials.
The Controller box should
contain; controller, power cord, 2.5mm screwdriver, 2mm Allen driver,
1/8” jack, and 3 pin mini DIN connector.
Check the packing list and call
your supplier if there are any discrepancies.
Bioptechs Objective Temperature Control System is comprised of two
components. One part is the
electronic controller unit; the other is an adjustable objective heater
loop which attaches to high numeric aperture (immersion) microscope
objectives. The objective
heater is designed to eliminate the heat-sink effect that is accelerated
by the presence of an optical coupling medium; oil, glycerin or water.
These objectives have a retractable central tube which contains
the optical elements. Heat
is directly transferred to this tube and sensed by a thermistor which is
part of an intelligent feedback control loop.
The heater loop requires a minimum of 5mm longitudinal physical
contact with the cylindrical objective surface.
In some cases there may be a decorative collar or sleeve on the
objective which must be removed in order to permit adequate surface
contact for the heater loop. If
an outer collar or sleeve is present we recommend using Bioptechs
Objective Wrench and Pliers for proper removal (Bioptechs Part Number:
021523; See figure 3
– outer sleeve removed).
PLEASE READ BEFORE USING THE BIOPTECHS, INC. OBJECTIVE HEATER!
are expensive and delicate. We
recommend following the directions below carefully. Although great care has been taken to produce a reliable
product to control the temperature of your objective, there is always
some risk. BIOPTECHS,
INC. assumes no responsibility for use of this or any other objective
heater. The user assumes all liability for any damage that may occur
due to the use of this objective heater.
Panel of Objective Heater Controller
Display should be illuminated when
the controller is turned on. If it does not light, check the main power source and the
fuse block located in the power input module where the AC power cord
Selector is the black button
adjacent to the display. It
selects the source of the information displayed.
When the controller is turned on, the default condition is to
display the setpoint value indicated by the red light adjacent to the
The button selects one of a sequence of display options.
This button can be pressed at any time and has no effect on the
temperature regulation functions of the controller.
Lights indicate the source of the
value displayed on the numeric display.
is adjusted by a potentiometer accessible with the 2.5mm screwdriver
provided. To adjust the
setpoint, set the numeric display to Setpoint
and adjust the potentiometer to the desired value.
If you need to change the setpoint value during your experiment
on a routine basis, there is a remote setpoint jack on the back of the
controller. See Remote
is the location where the
calibration of the circuit reading the objective thermistor can be
is the location where the
calibration of the circuit reading the reference probe can be adjusted.
(Red Button) resets the controller if the alarm sounds.
Note: If the alarm
went off there must have been a reason.
Check the objective before resetting the controller.
substitutes precision resistors into the circuit that reads the
thermistors so that calibration adjustments can be made to a known
connector is a six pin mini DIN connector where objective heater is
Panel of the Objective Controller
line input, power switch, voltage selector, and fuses are all
incorporated in the AC Power Input Module.
The controller can operate between 90 - 260V 50-60Hz, or a 24V
battery can be used as an alternate power supply.
The fuse carrier contains two 0.5A slow blow fuses.
The fuses can be accessed by prying the fuse block out of its
socket with a small screwdriver wedged under the small slot in the
opening of the AC line receptacle.
When replacing the fuse block, make sure to align the arrow on
the outside of the fuse block with the closest voltage value to your
supply line voltage:
For 90-130 supply line voltage
place the fuse block with the arrow pointing to 110-120V.
For 180-260 supply line voltage
place the fuse block with the arrow pointing to 220-240V.
Out is a voltage output direct from
two instrument amplifiers reading the thermistors from channel A and B.
The output from this
socket is temperature / 10. 37.0°C
= 3.70 Volt DC.
The left pin is channel
A, center pin is channel B, and right pin is ground.
Setpoint is an analog input that
allows the user to set the controllers setpoint from an external DC
source. The sleeve is earth ground. A DC voltage can be applied to the tip of this jack equal to
temperature /10. i.e.: 28.5°C
= 2.85 Volt. When the jack
is inserted into the controller it will automatically switch to the
jacks value. When removed
it will default to the setting on the front of the controller.
The remote setpoint can be used for programming or cycling
(The heater band should be unplugged from the controller and the
controller shut OFF)
Note: To reduce the possible detrimental effects of temperature cycling, it is recommended that the objective be kept in a warming box when not in use. We recommend the Boekel Warmer (Bioptechs Part Number: 260700)
Excessive use of immersion oil on an objective is detrimental to both the objective and the heater-band. Immersion oil should not be allowed to come into contact with the heater-band. If contact occurs the heater-band can be cleaned with a tissue dampened with alcohol.