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Boekel Desk Top Warmer When working with live cells on a
microscope it is convenient to have an incubator close to the scope. It
is beneficial for temporary storage of chambers (to keep them
pre-warmed), specimens or media used for perfusion, and most importantly
a clean, dry, safe place for heated objectives when not being used.
Bioptechs recommends the Boekel Warmer because its small size, light
weight. It is not humidified making it an ideal warmed objective storage
device. The warmer measures 8" x 8" x 7", operates on 20 watt AC and is
small enough to be mounted close to the microscope for temperature
controlled perfusion media storage during experiments. The incubators
temperature range is ambient +5°C to 42°C. Please specify 115v
(PN: 260700) or 230v (PN:
260700-2) when ordering. |

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Perfusion Tubing Bioptechs recommends using one of three
USP Class 6 certified tubings for maximum biocompatibility: PharMed
(available through Fisher, VWR and other suppliers), C-Flex, and Tygon
2275.
The main considerations for tubing used for cell culture and microscopy
are:
- Size
- Biocompatibility
- Gas permeability
- Autoclavability
- Transparency
Typically, 1/16th" inside diameter tubing is more than adequate to
perfuse the small volumes of cells that are used on a microscope. The
1/16th" diameter provides adequate flow and is easily workable. These
are the products that Bioptechs has found to be suitable for microscopy
applications. All have excellent biocompatibility and nearly zero gas
permeability. The main differences are the survivability after
autoclaving and transparency. Transparency is an issue when a parallel
plate chamber is used. If any bubbles form during the perfusion process
and get into the parallel plate optical cavity, they will adhere to the
glass and potentially ruin an experiment. If a transparent tubing is
used, the bubbles can be seen, giving you time to trap and eliminate
them before they do harm. The final issue is autoclaving. All three of
the top biocompatible tubings claim autoclavability. However, some
survive better than others. The following chart represents Bioptechs'
experience in testing these materials.
| Material |
Size |
Gas Permeability |
Transparency |
Autoclavability |
| C-Flex |
1/16" |
|
Translucent |
Excellent |
| Pharmed |
1/16" |
|
Opaque |
Unknown |
| Tygon 2275 |
1/16" |
|
Nearly Transparent |
Fair |
Part # 20202275 for Tygon 25ft
Part # 2020822 for C-Flex 50ft
- Bioptechs recommends and sells C-Flex tubing. It is available in
the 1/16" ID size and sold in 50 foot reels.
- C-Flex is a unique patented thermoplastic elastomer specifically
formulated to meet the critical demands of medical, and
pharmaceutical, research.
- C-Flex contains no toxic extractables (non-PVC, non-latex,
non-silicone)
- Complies with USP XXII, Class VI, FDA and USDA standards.
- Manufactured under strict GMP's in an FDA registered facility
- Significantly less permeable than silicone
- Low protein binding
- Ultra smooth inner bore
- Safely disposable
Additional specifications on C-Flex tubing are available through
Bioptechs |

Tygon 2275 Tubing Part# 20202275 (25 FT Roll)

C-Flex Tubing Part#
2020822 (50 FT Roll) |