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|Delta T® Open Dish System|
Finally a culture dish system specifically designed for live-cell microscopy! Now you can have accurate temperature control and high-numeric aperture compatibility in a convenient disposable culture dish system that even works in confocal applications.
The Bioptechs, Inc. Delta T Culture Dish System is designed to simulate host conditions on the stage of your microscope and provide an optimal optical environment for microscopy. This two-step system allows you to plate your cells and observe them without having to transfer them to another structure. The system components are, the Controller, a Stage Adapter, and Dishes. Accessories for Tissue Slice, Brain Slice, and other specimens are available.
An intelligent feedback loop passes an electrical current through a thin film coating on the underside surface of the glass substrate on which the cells are grown. Heat is applied directly to the cells without the inefficiencies associated with peripheral heating by traditional culture dish warmers. Bioptechs exclusively offers opaque culture dishes which eliminates the unwanted ambient light background for fluorescence imaging.
The controller features a real-time temperature display and fast learning curve to compensate for cooling due to surface evaporation while responding to temperature changes due to perfusion. There is also an alarmed protection circuit to safeguard the cells and an internal reference for the user adjustable calibration. The standard controller has a temperature range of ambient to 50° C. Extended ranges are available upon request.
Delta T dishes have 35mm O.D. and a 23mm central aperture. The peripheral region of the dish is tapered to reduce the dead-volume and the height of the dish is 6mm to allow better access for micro-injection and micromanipulation. The dishes are a hybrid of polystyrene plastic and Desag 263 glass. The outer structure of the dish is available in opaque black or clear and come with a clear 0.5mm or no.1.5 glass coverslip bottom bonded to it. The dishes are also available in a plain glass unheated version.
The thermograph on the left shows the efficiency, accuracy and uniformity of the Delta T system. Notice the temperature of the stage adapter. It is nearly the same temperature as the room temperature background. The dotted oval shows where the edge of the stage adapter is in visible light. Only the specimen and media are heated. Power consumption is 0.9 watts because heat is only applied to the specimen area. There is no heat transmitted to the stage. Therefore, it remains “Z” axis stabile. This is a sharp contrast to traditional peripheral heating methods (shown below right), and clearly superior.
The thermograph above on the right indicates the dis-advantage of peripheral heating. This is a thermal image of a 50mm culture dish in the center of a 100mm diameter uniformly heated, 3mm thick, aluminum plate with a 25 mm hole in the center. This image was acquired after 20 minutes of equilibration. Note the high temperatures of nearly 60° C, that it takes to reach 37° C in the specimen area. In this case heat that is not beneficial to the specimen is sunk into the stage causing Z-axis instability, not to mention the non-uniform temperature of the specimen area.
Perfusion in the Delta T
The illustrations below give you an overview on how to setup perfusion in the Delta T system, one drawing is for strictly CO2 perfusion above your cells. The second drawing depicts how to set up your Delta T System for perfusion with CO2 regulated media while perfusing your cells with a variety of perfusion devices. You can click on each image for a larger view.
Optional Accessories for the Delta T System:
Low numeric aperture users need only purchase the basic Delta T with .5mm dishes. However if you are using high numeric aperture objectives (immersion) you will need the 0.17mm dishes. It will be necessary to regulate the temperature of the objective as well. In this case a Bioptechs, Inc. Objective Heater and an Objective Heater Controller will be necessary for uniform temperature across the field. Once you become familiar with its operation, you will find the Delta T an indispensable addition to your scope.