How it works:

Cells are plated on a 40mm coverslip and placed into a special FCS2 chamber. This provides a perfuseable laminar flow optical chamber with user modifiable flow characteristics. The upper glass element (Microaqueduct slide) is then used to remove heat from the specimen cavity to be absorbed in the cooled fluid being circulated in the cavity formed by the addition of an O-ring sealed window adapter. Gravity or an electric pump is then used to create a flow of chilled fluid through the heat exchange cavity in the chamber. The cells remain safely enclosed in a separate optical enclosure. If more thermal transfer is necessary, tubing can be installed into the FCS2 base.

Note: If you are using high N.A. objectives at below ambient temperatures it will be necessary to cool and thermally isolate the objective from the scope. The optional thermal isolator will prevent condensation from forming on the lower element of the objective. A gas port is provided to purge the adapter with dry nitrogen. An anti-reflection coated window optimized for 340-700nm seals the bottom of the adapter.

The CFCS2 System is available in a few different configurations:

Cooled FCS2 Starter Set (includes:) Chamber with cooling tubes in base, 5 Non-coated Microaqueduct Slides, 50 Coverslips, 30 pc Gasket Set (Chamber has heating and cooling capability.) (PN: 03060319-2)

30mm Aperture Cooled FCS2 (CFCS2) includes: Chamber with a 30mm aperture and cooling tubes in base, 5 Non-coated Microaqueduct Slides, 50 Coverslips, 30 pc Gasket Set (chamber has heating/cooling capability.) (PN: 03060319-2-30).

Cooling Adapter for FCS2 sold separately (used for additional cooling of specimen region) (PN: 060319-2-0301)

Objective Cooling Collar

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The Objective Cooling Collar is an attachment to an objective that provides an isolated pathway through which a refrigerated fluid can flow. this provides an efficient means of cooling the objective. The source of the chilled fluid can be as simple as a dewar of ice water or as sophisticated as an AC powered chiller bath. The removal of heat from the objective is more difficult than heating because you are limited by the thermal transfer efficiency of both the objective and the contact surface of the cooling ring. Therefore, the cooling ring needs to be of sufficient size to work. It is advisable to make sure you have enough room under the stage for the additional diameter required for the cooling collar and tubing. Cooling collars are precision machined to fit specific objectives. Please call Bioptechs for additional compatibility information.
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