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When imaging cells in a rigid structure such as a chamber
having a coverslip as an observation surface, one must appreciate the fact that
any rapid changes in flow rate will translate to microdynamic changes in
pressure within the optical cavity of the chamber. This causes the coverslip to
behave like a diaphragm thus flexing out of focus due to the narrow depth of
field of the microscope objective. To eliminate this problem at the lower flow
rates and significantly reduce it at higher flow rates, Bioptechs recommends the
Micro-Perfusion pump for use with all its micro-observation systems.
The Micro-Perfusion Pump is a miniature, single or dual, channel, full-featured
peristaltic pump designed specifically for low-flow rates. Unlike most
peristaltic pumps that are driven by stepper motors, the Micro-Perfusion pump is
driven by a tachometer regulated, multi-stage DC gear motor. This assures a
smooth analog rotation of the roller spindle, free of instantaneous steps. It is
regulated by either the internal control circuitry adjustable from 0.2-180 ml/hr
(dependant on pump)
or it can be interfaced with a computer through a DAIO port. Bioptechs provides
a kit for this purpose that includes all cabling, software, and hardware needed
for a dual pump setup. The pump comes with an external 9 volt AC adapter and
also contains an internal 9 volt battery which can function as the primary power
supply if needed. Multiple tubing sizes are available, 0.062" is generally best suited
for imaging applications. The pump tubes are available in silicone rubber and
C-Flex. They are terminated with a 1/16" tubing barb. The base of the pump is
threaded for easy mounting to a stand or fixture near the microscope.
Features
Compact
Usable as either single or dual channel
Precise speed setting feature
Flow rates of 0.2 to 180 ml/hr
Compatible with 1/16" tubing
External DC power supply with internal 9 volt backup battery
Can be operated on internal battery if desired
Computer interface available
Tubing Sizes and Flow Rates for Single
Tube Set:
|
Tubing ID |
Normal Pump |
Low-Flow Pump |
High-Flow Pump |
Part # (C-flex) |
|
.015" |
0.2 - 5 ml/hr |
0.02 - 0.45 ml/hr |
2 - 43 ml/hr |
060319015C15 |
|
.020" |
0.4 - 11 ml/hr |
0.04 - 0.95 ml/hr |
4 - 90 ml/hr |
060319020C20 |
|
.031" |
0.8 - 21 ml/hr |
0.08 - 1.8 ml/hr |
9 - 170 ml/hr |
060319031C31 |
|
.062" |
3 - 75 ml/hr |
0.3 - 6.3 ml/hr |
33 - 550 ml/hr |
060319192016 |
Pump Descriptions and Part Numbers:
| Description |
Part Number |
| FCS2 & FCS3 Micro-Perfusion Pump (Normal) |
060319131616 |
| FCS2 & FCS3 Micro-Perfusion Pump (Low-Flow) |
0603191310683 |
| FCS2 & FCS3 Micro-Perfusion Pump (High-Flow) |
0603191372066 |
| Delta T Micro-Perfusion Pump (Normal) |
0420131616 |
| Delta T Micro-Perfusion Pump (Low-Flow) |
04201310683 |
| Delta T Micro-Perfusion Pump (High-Flow) |
04201372066 |
|