What is it?
The Perfusion Temperature Control interface is a computer program with associated interface hardware that allows the user to intuitively and repeatedly control perfusion or temperature in micro-fluidic cell chambers for time lapse imaging. It is designed to address the fluidic and temperature control issues typical of chemically or thermally induced change experiments in live-cell chambers. The program provides a WYSIWYG (What You See Is What You Get) graphic preview of your input values then executes the values on command. Requires two Bioptechs Micro-perfusion pumps.
|
Why would I need this or what value would it give my experiments? The greatest value, other than its utility, is the fact that you can save and repeat settings so that statistically comparative data can be acquired. It is extremely ease to use ( just fill in the blanks) for precise and repeatable control of:
This program is a real time saver and valuable tool for anyone needing automated control of Bioptechs Micro-perfusion pumps. |
|
How does it work?
Profiled Flow Cycle
A typical profiled flow scenario: Ca++ free medium flows from pump [A]
at a specified low flow rate while cells are imaged to demonstrate their
initial state for a selected period of time. Then pump [A] is shut off
as Ca++ rich medium from pump [B] starts at the same flow rate as the
previous [A]. Pump [B] then accelerates at a specified rate to a
specified peak flow rate and holds for a specified period of time
sufficient to displace the dead volume and diffusion gradient of the
prior media in the tubing. At this time the flow rate of pump [B] is
reduced to a specified rate optimal for imaging. This process is then
repeated either cyclically or on demand. In this manner you have the
ability to produce a quick, reliable and repeatable change of media.
Alternating Flow Cycle
An alternating flow scenario is used when the speed of transition and the need
for a reduced flow rate for imaging is not necessary: This mode also enables the
selection of a starting flow rate that is not timed so that the user can
initiate the alternating flow upon demand. When start cycle is clicked the pumps
simply alternate at a user specified flow rate and time duration without
profile.
Temperature Cycling
The PTC program provides an intuitive graphic interface to program temperature
variations into Bioptechs micro-environmental systems.
There is no typical scenario for temperature variations. However, the program
provides three stages of temperature transition per cycle and a terminal or
infinite number of cycles.
|
If you would like to demo this program, all you have to do is download the zipped program and uncompress the folder. There is no need to install the program to test it, simply run the Bioptechs Controller.exe direct from the uncompressed folder. Once the program starts it will ask you for a serial number, hit cancel and the program will be in demonstration mode so you can simulate experiments. When the program is in demonstration mode the program will not attempt to send values to a piece of hardware. |
 Click to Download |
Program Screen Shots
|
Profiled Flow Window: |
Temperature Control Window: |
|
Alternating Flow Window: |
Self Help Wall Chart: This self help wall chart will give you an in-depth look at the program interface with descriptions for each button, function tab, and user input box for the profiled flow and temperature control tabs. A full size 21 inch by 24 inch laminated self help wall chart is included when purchased. |
1 program CD
1 program license key
1 user instruction manual
1 laminated self help wall chart
1 USB D/A
interface box
1 cable to control two micro-perfusion pumps or one Bioptechs
temperature controller
1 cable to read data from a Bioptechs temperature
controller
|
E-mail: info@bioptechs.com |