Check List for Ordering a Delta T Culture Dish System

Having established the need for an open dish system, there are a few technical matters that should be considered. Following this guide will ensure that these areas have been addressed.

When Ordering a Delta T System:

1. Provide the microscope manufacturer’s name and model of stage to identify the appropriate stage adapter.

2. Indicate the type of specimen.

1. In the case of adherent cultured cells and low N.A. objectives, you will only need the basic Delta T4 System. It is comprised of a Delta T4 Dish Controller and a Stage Adapter plus a suitable quantity of 0.5mm dishes.
2. If the specimen is a natural membrane, cut section, or tissue slice, an appropriate specimen carrier will need to be added to the basic system. (see Tissue Slice, Brain Slice, and Artificial Membrane pages).

3. Determine if high or low N.A. lenses will be used. Order the appropriate dishes for the N.A. of the objectives.

• For low N.A. applications, order 0.5mm thick bottomed dishes, Bioptechs PN 04200405
• For high N.A. applications, order #1.5 coverslip bottomed dishes, Bioptechs PN 042004015

Note: If the application involves fluorescence, add the suffix B to specify black sidewall dishes. Clear dishes are shipped with clear lids & black dishes are shipped with black lids.

4. If the cells need perfusion, the following items should be included on the order:

5. If contrast enhancement techniques are used for transmitted images on an inverted microscope, consider the option of a Heated Lid. Bioptechs PN 04200803

6. If a series of such images are to be made into a movie loop, the Coverglass Lid, with or without perfusion, is advisable.

7. If high numeric aperture objectives are to be used, you will need to order the following:

We cannot guarantee that our stock Objective Heaters fit all objectives. Therefore we ask that you specify the make, magnification, N.A. and any other attributes of the objectives you intend to use. This step will assist us in determining the compatibility of our Objective Heater with your optics.

8. If the temperature of the specimen needs to be below ambient, include the Delta T Cooling Ring attachment. Bioptechs PN 04200318