Check List for Ordering an FCS2

Having established the need for a closed system, there are a few technical matters that shouldbe considered. Following this guide will ensure that these areas have been addressed.

The FCS2 is used for both high and low numeric aperture applications. Therefore, the objective heater and objective temperature controller are sold separately. The main optional consideration is perfusion.

When Ordering an FCS2 System:

1. Select a stage adapter from the FCS2 stage adapter page and provide the microscope manufacturer’s name and model of stage to confirm identification of the appropriate adapter.

2. If the cells need perfusion, the following items should be included on the order:

3. Although the starter set comes with one ounce of 40mm coverslips, you may want to order additional coverslips. Bioptechs PN 40-1313-0319

4. If high numeric aperture objectives are to be used, you will need to order the following:

We cannot guarantee that our stock Objective Heater fits all objectives. Therefore we ask that you specify the make, magnification, N.A. and any other attributes of the objectives you intend to use. This step will assist us in determining the compatibility of our Objective Heater with your optics.

5. Indicate the N.A. of the transmitted condenser if it will be used.

6. Supplemental components for FCS2 operation at temperatures below ambient are:

• FCS2 Cooling Adapter (fits all FCS2s) Bioptechs PN 060319-2-0301

• Objective Cooling Collar Bioptechs PN 150303 (individually machined to fit user’s own objective)

• Objective Thermo-Isolator Bioptechs PN 152009 (with compensating lens specific to tube length of objective)