Closed Chamber Systems

Closed systems are used when the experiment requires that the specimen be contained in a completely air tight environment or the optical constraints of the microscope dictate a more precise definition of optical surfaces. The following section offers integrated solutions for closed-system micro-environmental control. All of these systems are optically compatible with nearly all modes of light microscopy, have near laminar flow, and user definable volume and flow characteristics. Models available for both upright and inverted microscopes.

Open Dish Systems

The Bioptechs Delta T™ open dish system uses glass bottomed dishes and accommodates a wide variety of specimen types. This system is ideal for multi user facilities or those that are undecided about which chamber type is required. It is ideal as an open dish system for applications such as: micro-manipulation and micro-injection, electro-stimulation, wounding, recording, and chemical stimulation. The Delta T™ has an assortment of convenient accessories that optimize its use as a perfusable or non-perfusabe closed system chamber for adherent cells requiring control of CO2, and humidity or provide a second optical surface to equalize contrast for time-lapse imaging. There are also special supports available for natural and artificial membranes. Short of needing a defined flow for perfusion, the Delta T™ is the most convenient means of micro-environmental control for a microscope.

	Delta T for Inverted or Upright Scopes Delta T Stage Adapters Demonstration Video
	Optional Accessories for the Delta T Artificial Membrane Adapter, Atmospheric Barrier Ring, Brain Slice Adapter, Cooling Ring, Coverglass Lid, Culture Cylinders,  Coverslip Weight, Heated Lid, Hinged Perfusion Adapter, Media Depth Reducer, Tissue Slice Adapter, Yeast Slide Adapter
	Disposable Culture Dishes Self Heating Dishes Non-Heating Dishes
	EDU System
	Stable Z System

Objective Heating or Cooling Systems

When using high-numeric aperture lenses, heat is lost very rapidly from the specimen through the optical coupling medium (oil or water) to the objective. Control of the objective temperature eliminates this problem.

	Objective Heater System Objective Sizing Page Demonstration Video
	Objective Options Cooling Collar, Thermal Spacer, Thermal Isolator, Wrench and Pliers

Perfusion Devices

The perfusion products in this section have been carefully chosen or developed to address the specific fluidic requirements for doing time lapse imaging of live cells. Here you will find dedicated tried and proven perfusion systems to make your time-lapse imaging easier.

	Micro Perfusion Pump Rod Mounting Clamp, Tubing Sets
	Computer Controlled Perfusion Demonstration Video
	CO2 Metering Pump

Accessories

	C-Flex and Tygon 1/16" Perfusion Tubing
	1/16" Perfusion Adapter Set
	Boekel Warmer