Delta T

dt_starter_set1
Black dish w/ lid closeDelt T ziess GDelta T Galv RectDelta T Galv RoundDelta T Marzhouser ScanDT Controller backDT Controller bottomDT Controller componentsDT Controller frontDT Controller Iso tempDT Controller leftK T5 topM T5 top no dishNODR T5 topSLAS-SBS T5UU T5 top no dish

The Bioptechs Delta T™ System is a micro-environmental control system used on microscopes to enable long term acquisition of high resolution, time-lapse images of cells.  The Delta T™ is designed to simulate host conditions on the stage of a microscope and provide an optimal optical environment for microscopy. This two-step system enables cells to be plated and observed without having to transfer them to another structure for imaging.   The basic system components are, the Controller, a Stage Adapter, and Dishes. There are a variety of accessories for the Delta T™ that enable it to be used with nearly any specimen type.

 

0420-4 DELTA T™ STARTER SET $4,958.17


The Bioptechs Delta T™ Culture Dish System is the only culture dish that provides heat to the contents of the dish without heating the stage of the microscope. It uses number 1.5 thickness cover glass bottomed dishes that have an electrically conductive, optically transparent coating of ITO on the underside.  The coating is electrically powered to directly warm the contents of the dish.  This is an exclusive technology to Bioptechs and provides features and capabilities no other system can match. An intelligent feedback loop passes an electrical current through a thin-film coating on the underside surface of the glass substrate on which the cells are grown. Heat is applied directly to the cells without the inefficiencies associated with peripheral heating by traditional peripherally heated culture dish warmers. Bioptechs exclusively offers opaque culture dishes which eliminates the unwanted ambient light background for fluorescence imaging.  The controller features a real-time temperature display and fast learning curve to compensate for cooling due to surface evaporation while responding to temperature changes due to perfusion. There is also an alarmed protection circuit to safeguard the cells and an internal reference for the user adjustable calibration. The standard controller has a temperature range of ambient to 50° C. Extended ranges are available upon request.

Delta T™ dishes have 35mm O.D. and a 23mm central aperture. The peripheral region of the dish is tapered to reduce the dead-volume and the height of the dish is 6mm to allow better access for micro-injection and micromanipulation. The dishes are a hybrid of polystyrene plastic and Desag 263 glass. The outer structure of the dish is available in opaque black or clear and come with a clear 0.5mm or No. 1.5 glass coverslip bottom bonded to it. The dishes are also available in a plain glass unheated version.It accommodates a wide variety of specimen types including adherent cells, suspended cells, tissue, and artificial membranes. This system is ideal for multi-user facilities or those that don’t need a flow cell but are undecided about what type of chamber system is required. The Delta T™ has an assortment of convenient accessories that expand its capabilities.  The most often used accessories are the Heated Lid, Coverglass Lid, and Culture Cylinders.  Short of needing a defined flow for perfusion, the Delta T™ is the most convenient, accurate, means of micro-environmental control for a microscope.

Delta T dish dimensionsController FeaturesBioptechs Delta T vs. Traditional Stage Heating

Delta T
Delta T
Every Other Traditional "Stage Heater"
Every Other Traditional "Stage Heater"

  • Easier technique

  • Accurate temperature control

  • 
Low dead volume

  • 
Fast recovery

  • Superior optical image


  • Heating stages systems are not efficient

  • Plastic dishes are a poor conductor of heat

  • Temperature does not recover quickly during or after perfusion

  • 
Plastic bottomed dishes are not suitable for high resolution microscopy

  • Non-uniform temperature distribution

  • Unnecessary dead volume

 

Thermographic Images
The thermograph on the left shows the efficiency, accuracy and uniformity of the Delta T™ system. Notice the temperature of the stage adapter. It is nearly the same temperature as the room temperature background. The dotted oval shows where the edge of the stage adapter is in visible light. Only the specimen and media are heated. Power consumption is 0.9 watts because heat is only applied to the specimen area. There is no heat transmitted to the stage. Therefore, it remains “Z” axis stable. This is a sharp contrast to traditional peripheral heating methods (shown below right), and clearly superior.

Delta T Thermographic Image
Delta T Thermographic Image
Radiant Stage Heater Thermographic image
Radiant Stage Heater Thermographic image

The thermograph above on the right indicates the disadvantage of peripheral heating. This is a thermal image of a 50mm culture dish in the center of a 100mm diameter uniformly heated, 3mm thick, aluminum plate with a 25 mm hole in the center. This image was acquired after 20 minutes of equilibration. Note the high temperatures of nearly 60° C, that it takes to reach 37° C in the specimen area. In this case heat that is not beneficial to the specimen is sunk into the stage causing Z-axis instability, not to mention the non-uniform temperature of the specimen area.

Delta T Gas Perfusion Diagram

Bioptechs Delta T Gas Perfusion Diagram


Ideal for first time users and multi-user facilities. Bioptechs has combined the Delta T with the three most commonly used accessories to get you up and running as fast and trouble free as possible at a reduced price.The Bioptechs starter set will immediately give you the micro-environment base for your cells to start high end time lapse microscopy with the ease of disposable culture dishes, and the versatility to image a wide variety of cell and tissue cultures directly out of the box.

The starter set includes:

  • (1) Delta T™ Controller
  • (1) 10pk of Delta T™ Dishes 0.17mm thick (clear unless specified)
  • 
(1) Coverglass Lid
  • 
(1) Heated Lid with CO2 port
  • (1) Culture Cylinder Pack, (1) 4mm, (1) 6mm, (1) 8mm, (1) 10mm
  • 
(1) Standard Stage Adapter, please specify when ordering

 

  • Easily adapted to a wide variety of specimen types from monolayered adherent cells to brain slice and tissue preps
  • Low mass to thermo-regulate as opposed to conventional stage heaters
  • Plate, incubate, and observe without the need to transfer your cells
  • Fast thermal recovery after perfusion (within seconds)
  • Compatible with inverted and upright microscope stands (Stage Adapters)
  • Coverglass bottom for optimum optical compatibility
  • No need for warm air blowers or stage heaters
  • Direct first-surface heating to your cells
  • Can also be cooled below ambient
  • Perfusion available (Perfusion Configuration)
  • No preheating

 

Delta T Culture Dish System
Delta T Starter Set 0420-4 $4,958.17
Includes the following: Delta T Controller, Heated Lid, Coverglass Lid, Culture Cylinder Pack: (1) 4mm, (1) 6mm, (1) 8mm, (1) 10mm,    
0.17mm clear dishes (10/pk), Standard Stage Adapter (please specify which stage when ordering)
Delta T Culture Dish Controller 0420-4-03 $3,731.75
Controller Power Supply 19-01 $37.95
Delta T Culture Dish Standard Stage Adapters
Leica DMIRB Triple Plate Adapter 04201203 $820.99
Nikon, Olympus, Deltavision Round Adapter 04201415 $820.99
96-Well, SBS Plate Sized Adapter 04202003 $820.99
Upright Universal Adapter 04202116 $820.99
Marzhauser, Zeiss (K), ASI, Ludl & Prior Adapter 04202602 $820.99
Zeiss (M) & Leica Single Plate Adapter 04202601 $820.99
Delta T Culture Dish Custom Stage Adapters
Zeiss (G) Adapter 04202603 $1,119.53
Marzhauser Scan 100×80 (160×116) 1917 $1,119.53
Leica Galvanic Round Adapter 04201207 $1,119.53
Leica Galvanic Rectangular Adapter 04201807 $1,119.53
Marzhauser Galvanic Rectangular Adapter 04202807 $1,119.53
Quixell Adapter 4201703 $1,119.53
Delta T Culture Dishes (10/pk)
0.5mm thick glass (clear) 0420042105C $66.13
0.5mm thick glass (black) 0420042105B $66.13
0.17mm thick glass (clear) 04200417C $66.13
0.17mm thick glass (black) 04200417B $66.13
Delta TPG Culture Dishes (10/pk)
0.5mm thick glass (clear, no temperature control) 0420040500 $40.25
0.17mm thick glass (clear, no temperature control) 0420041500C $40.25
0.17mm thick glass (black, no temperature control) 0420041500B $40.25
Discount Price for Multiple Quantities of Delta T Dishes   25 to 49 pk’s = 5% discount, 50+ pk’s = 10% discount
Delta T Culture Dish Accessories
Tissue Slice Adapter 0420201918 $522.45
Artificial Membrane Adapter 0420011301 $597.08
Brain Slice Adapter 0420201919 $597.08
Hinged Perfusion Adapter Set (2/set) 0420081601 $447.81
Cooling Ring 04200318 $223.91
Heated Lid 04200803 $447.81
Perfusable Heated Lid 0420080316 $559.08
Replacement Silicone O-rings for Heated Lids (5/pk) 40-1350SS $34.87
ACBR 04200325 $74.64
Coverglass Lid 04200312 $223.91
Perfusable Coverglass Lid 0420031216 $373.18
Media Volume Reducer 04200303 $469.05
22mm Coverslip, #1.5 Thick, 100 Count 0420-0323-2 $44.78
22mm Coverslip Weight 0420-0323-1 $44.78
22mm Glass Spacer (for Coverslip Weight) 070303-22-2 $34.87
Perfusion
Delta T Micro-Perfusion Pump 0420131616 $1,195.00
Delta T Micro-Perfusion Pump (Ultra Low-Flow) 04201310683 $1,350.00
Delta T Micro-Perfusion Pump (High-Flow) 04201372066 $1,350.00
Delta T Double Tube Pump Set (4/set) C-Flex (0.062″) 0420042016 $137.50
Delta T Double Tube Pump Set (4/set) Silicon (0.062″) 0420042016S $137.50
Delta T Double Tube Pump Set (4/set) C-Flex (0.031″) 042004031C31 $137.50
Delta T Double Tube Pump Set (4/set) C-Flex (0.020″) 0420020C20 $137.50
Delta T Double Tube Pump Set (4/set) Silicon (0.015″) 0420015S15 $137.50
C-Flex Tubing (50ft) 202050 $62.50
Micro-Perfusion Pump Rod Mounting Clamp 16181303 $37.95
CO2 Metering Pump 03152-16 $600.00
Micro Gas Humidifier 130708 $650.00
CO2 Gas Regulation Valve (Demand Regulator) 03152-1822 $600.00
 Oasis Heating & Cooling System (2 to 45 C)  15-160  $3,125.00
 Perfusion / Temperature Controller (USB Interface)  13161603-13  $3,680.00