FCS Perfusion

Pump 6
Pump 2PumpPump 4Rod Mounting Clamp on pumpPump with single tube 2Single tubeRod Mounting Clamp

The Micro-Perfusion Pump is a miniature, single or dual, channel, full-featured peristaltic pump designed for specific ranges of flow rates conducive to microscopy. Unlike most peristaltic pumps that are driven by stepper motors, the Micro-Perfusion pump is driven by a tachometer regulated, multi-stage, DC gear motor. This assures a smooth analog rotation of the roller spindle, free of instantaneous steps.

60319131616 FCS2 MICRO-PERFUSION PUMP LOW FLOW $1,195.00
603191310683 FCS2 MICRO-PERFUSION PUMP ULTRA LOW FLOW $1,350.00
603191372066 FCS2 MICRO-PERFUSION PUMP HIGH FLOW $1,350.00

 



The Problem:
When imaging cells in a rigid structure such as a chamber having a coverslip as an observation surface, one must appreciate the fact that any rapid changes in flow rate will translate to micro-dynamic changes in pressure within the optical cavity of the chamber. This causes the coverslip to behave like a flexible diaphragm which causes the specimen to go out of focus with each step.


The Solution:
To eliminate this problem at the lower flow rates and significantly reduce it at higher flow rates, Bioptechs recommends the model 720 Micro-Perfusion Pump for use with all its micro-observation systems. This Micro-Perfusion Pump is a miniature, single or dual, channel, full-featured peristaltic pump designed specifically for low-flow rates. Unlike most peristaltic pumps that are driven by stepper motors, the 720 Micro-Perfusion Pump is driven by a tachometer regulated, multi-stage DC gear motor. This assures a smooth analog rotation of the roller spindle, free of instantaneous steps. It is regulated by either the internal control circuitry adjustable from 0.8 µL/min to 18 ml/min (dependant on pump model) or it can be interfaced with a computer through a USB D/A port. The pump comes with a 9 volt AC adapter and also contains an internal 9 volt battery which can function as the primary power supply if needed. Multiple tubing sizes are available, from 0.015 to 0.093, while 0.031″ is generally best suited for most imaging applications. The pump tubes are available in silicone rubber and C-Flex. They are terminated with a 1/16″ tubing barb. The base of the pump is threaded for easy mounting to a stand or fixture near the microscope. An optional rod mounting clamp is available for mounting to a 1/2″ rod.Bioptechs has developed a perfusion control system that provides cabling, software, and hardware needed for controlling the flow profile of two pumps typically used for induced change experiments. See (Perfusion Temperature Control)
Tube sets for in the pump need to be ordered separately. Tube sets are not included with the pump. Please see the price list for part numbers and pricing of tube sets and pumps.
Click here for a PDF chart of flow rates

Micro-Perfusion Pump Single Tube Set (4/set)
Single tubeThe Micro-Perfusion Pump Single Tube Set – single channel peristaltic tubing made of C-Flex for optimal durability. Also available in silicon. See price list for part numbers.


Micro-Perfusion Pump Double Tube Set (4/set)
Double tubeThe Micro-Perfusion Pump Double Tube Set – double channel peristaltic tubing made of C-Flex for optimal durability. Also available in silicon. See price list for part numbers.


Micro-Perfusion Pump Rod Mounting Clamp
Rod Mounting ClampThe Micro-Perfusion Pump Rod Mounting Clamp provides a method of mounting perfusion pumps onto conventional 1/2″ support post. Makes positioning perfusion pumps adjacent to the microscope easy and convenient.


Need help or not sure how to setup your perfusion system? Simply click on the images below.

Delta T Perfusion (2D)
Delta T Perfusion (2D)
FCS2 Perfusion (2D)
FCS2 Perfusion (2D)

  • Compact
  • Usable as either single or dual channel
  • Precise speed setting feature
  • Flow rates of 0.8 µL/min to 18 ml/min (pump dependant)
  • Compatible with 1/16″ tubing
  • Universal ?VDC adapter that accepts an input of 100 – 240VAC. Power supply has plugs for US, Europe, UK, and Australia
  • 9V battery backup
  • Can be operated on internal battery if desired
  • Computer interface available, (See Perfusion Temperature Control)

Tubing I.D. Ultra Low Flow Pump Low Flow Pump High Flow Pump Part # (C-Flex) Single Tube Set
0.015″ 0.8 – 7.5 µL/min 8 – 83 µL/min 0.07 – 0.7 ml/min 060319015C15
0.020″ 1.6 – 16 µL/min 18 – 180 µL/min 0.15 – 1.5 ml/min 060319020C20
0.031″ 3 – 30 µL/min 35 – 350 µL/min 0.3 – 2.8 ml/min 060319031C31
0.062″ 10 – 100 µL/min 125 – 1250 µL/min 0.9 – 9 ml/min 60319192016
0.093″ 20 – 200 µL/min 240 – 2400 µL/min 1.8 – 18 ml/min 060319093C93


FCS2 System
FCS2 Starter Set (includes:) 060319-2 $5,970.80
FCS2 Chamber, Chamber Controller    
5 Microaqueduct Slides, 40mm Coverslip (100 pk),    
and Gasket Set (30/set)
FCS2 Chamber 060319-2-03 $2,462.96
FCS2/3 Chamber Controller 060319-2-0303 $2,985.40
Controller Power Supply 19-01 $37.95
No-Heat Flow Cell (FCS2) 03060319-2-NH $2,686.86
Includes: Non-heated FCS2 Chamber, (5) Non-heating Microaqueduct Slides, (50) #1.5 – 40mm Coverslips, (30 piece) gasket set
FCS2 Stage Adapters
Leica DMIRB Triple Plate Adapter 060319-2-12 $820.99
Nikon, Olympus, Deltavision Round Adapter 060319-2-1415 $559.77
Nikon Round Adapter (Nikon Only) 060319-2-15 $559.77
Marzhauser Slim Stage 100×80 (160mmx116mm) 060319-2-1219 $1,119.53
Marzhauser, Zeiss (K), ASI, Ludl & Prior Adapter 060319-2-2611 $970.26
SBS / 96 Well Plate Adapter 060319-2-2003 $948.75
Zeiss (M) & Leica Single Plate Adapter 060319-2-2613 $746.35
Mad City Labs Stage Adapter 060319-2-13319 $1,119.53
FCS2 & FCS3 Supplies
Microaqueduct Slides (5/pk) 130119-5 $522.45
40mm Coverslips (200 pk) 40-1313-0319 $241.61
40mm Coverslips (500 pk) 40-1313-03192 $522.45
40mmCoverslips (1000 pk) 40-1313-03193 $970.26
FCS2/3 Gasket Set (30/set) 060319-2-0719 $223.91
30mm Round w/holes 0.75mm Thick – 5/pack 1907-08-750 $37.32
Blank Gasket .1mm Thick – 5/pack 1907-100 $37.32
Blank Gasket .25mm Thick – 5/pack 1907-250 $37.32
Blank Gasket .5mm Thick – 5/pack 1907-500 $37.32
Blank Gasket .75mm Thick – 5/pack 1907-750 $37.32
Blank Gasket 1mm Thick – 5/pack 1907-1000 $37.32
14 x 24 Rectangle Gasket .1mm thick – 5/pack 1907-1422-100 $37.32
14 x 24 Rectangle Gasket .25mm thick – 5/pack 1907-1422-250 $37.32
14 x 24 Rectangle Gasket .5mm thick – 5/pack 1907-1422-500 $37.32
14 x 24 Rectangle Gasket .75mm thick – 5/pack 1907-1422-750 $37.32
14 x 24 Rectangle Gasket 1mm thick – 5/pack 1907-1422-1000 $37.32
30mm Round Gasket .1mm thick – 5/pack 1907-18-100 $37.32
30mm Round Gasket .25mm thick – 5/pack 1907-18-250 $37.32
30mm Round Gasket .5mm thick – 5/pack 1907-18-500 $37.32
30mm Round Gasket .75mm thick – 5/pack 1907-18-750 $37.32
30mm Round Gasket 1mm thick – 5/pack 1907-18-1000 $37.32
Custom Gasket (5pk) Die # on Guide $37.32
FCS2 Accessories
FCS2 Cooling Adapter 060319-2-0301 $597.08
Open Mode Adapter 060319-2-1513 $260.54
FCS2 Low Dead Volume Top 060319-2-1242 $738.89
Cooled FCS2 (CFCS2) 03060319-2 $3731.75
Perfusion
FCS2 Micro-Perfusion Pump (Low-Flow) 060319131616 $1,195.00
FCS2 Micro-Perfusion Pump (Ultra Low-Flow) 0603191310683 $1,350.00
FCS2 Micro-Perfusion Pump (High-Flow) 0603191372066 $1,350.00
FCS2 Single Tube Pump Set (4/pk) C-Flex .062″ ID 060319192016 $137.50
FCS2 Single Tube Pump Set (4/pk) Silicon .062″ ID 060319192016S $137.50
FCS2 Single Tube Pump Set (4/pk) C-Flex .031″ID 060319031C31 $137.50
FCS2 Single Tube Pump Set (4/pk) C-Flex .020″ ID 060319020C20 $137.50
FCS2 Single Tube Pump Set (4/pk) Silicone .015″ID 060319015S15 $137.50
C-Flex Tubing (50ft) 202050 $62.50
1/16″ Perfusion Adapter Set 162003-1 $28.75
Micro-Perfusion Pump Rod Mounting Clamp 16181303 $37.95
CO2 Metering Pump 03152-16 $600.00
Oasis Heating & Cooling System (2 to 45 C) 15-160 $3,125.00
Perfusion / Temperature Controller (USB Interface) 13161603-13 $3,680.00
FCS3 System
FCS3 Starter Set (includes:) 21-060319-3 $5,970.80
FCS3 Chamber, Chamber Controller,    
, 5 Microaqueduct Slides, 40mm Coverslip (100 pk),    
Gasket Set (30/set), Spanner Wrench
FCS2/3 Chamber Controller 060319-2-0303 $2,985.40
Controller Power Supply 19-01 $37.95
FCS3 Non-Heating Chamber System Starter Set 21-060319-3NH $2,686.86
Includes: Non-Heated FCS3 Chamber, (5) Non-Hetaing Microaqueduct Slides, (50) #1.5 – 40mm Cloverslips, (30 piece) gasket set
FCS3 Stage Adapters
FCS3 Universal Upright Stage Adapter 21-060319-3-08 $1,492.70
FCS3 Marzhauser Scan 100×80 (160×116) 21-060319-3-2601 $1,865.88
FCS3 Marzhauser / Zeiss K Stage Adapter 21-060319-3-2611 $1,865.88
FCS3 Supplies
FCS3 Chamber 21-060319-3-03 $2,388.32
FCS3 Controller 21-060319-3-0303 $2,985.40
FCS3 Spanner Wrench 21-060319-3-1923 $80.61
FCS3 Pressure Plate 21-060319-3-16 $53.74