FCS2 System

FCS2 Starter Set2
40mm cvr slips gloveCFCS2 wht angle1FCS2 Chmb trans bkFCS2 Chmb trans bkFCS2 Chamber 8FCS2 Controller frontFCS2 Controller btmFCS2 Controller

The FCS2® System is a closed system, parallel plate flow cell environmental chamber used on a microscope to enable the acquisition of high resolution, time-lapse images of cells undergoing either natural growth or fluidic exposure to variant factors.  The FCS2® System is designed to be used on any inverted microscope as the FCS3® System is specifically for upright microscopes.  Both systems enable the user to define precise and unlimited flow characteristics within the chamber to suit their protocols.  Bioptechs’ patented thermal design provides uniform temperature control across the entire field.  This is a feature that is not possible with a peripheral only heated chambers.

 

060319-2 FCS2 STARTER SET $5,970.80


What is it?
The Focht Chamber System 2 (FCS2®) is a closed system, live-cell micro-observation chamber that utilized several patented technologies that offer several exclusive advantages over other chambers. In addition to its uniform temperature control and user definable perfusion capability, it is fully compatible with all modes of microscopy. It is also the only chamber to combine high-volume laminar flow perfusion rates with Koehler illumination and precise temperature control without the need for an air curtain. This is accomplished by the development of micro aqueduct perfusion. A method of introducing media into a fluid optical cavity where the separation of optical surfaces and flow geometry is definable by the user. Therefore, the chamber is adaptable to the protocol instead of having to adapt the protocol to the chamber.

The FCS2 is a flow cell or flow chamber that is designed specifically for the demands of today’s live-cell imaging requirements. It has limitless flow characteristics because its flow geometry can easily be customized by the user. It provides the ability to configure the optical cavity to produce control shear forces, flow characteristics, high or low volume exchange rates, near laminar flow, and anything in between. The most important feature of the FCS2, other than unequalled temperature uniformity, is that the is flow of media is constrained to a precise location over the cells. Open dish type chambers do not provide the flow capabilities of the FCS2. The FCS2 provides unmatched, uniform temperature control, in a user configurable, perfusable, optical imaging cavity.

FCS2 users enjoy the following characteristics:

  • Easy no tools needed assembly
  • Compatibility with all modes of microscopy
  • Complete control of the volume within the optical cavity
  • Complete control of the separation between optical surfaces
  • Complete control of the shape or profile of the flow channel
  • Complete control of the temperature uniformly across the entire field
  • Compatible with inverted microscopes


How does it work?
Cells are grown on a 40mm glass coverslip. This coverslip is then incorporated into a perfusable fluid optical cavity that is compatible with all modes of microscopy, and its geometry can be easily defined by the user. This optical cavity is secured into a fixture on the stage of the microscope where it can be perfused with media or remain static. Media that comes into one of the ports on the side of the chamber, emerges in a fluid optical path where the media is precisely directed over the cells. The media is collected within the optical cavity and directed out of the chamber on the other side. The flow characteristics of the media while in the optical cavity are easily modified by selecting gaskets of varying geometry that direct the flow. Temperature control is maintained by both peripheral thermal support from the chamber’s metal frame and heat that is emitted from an electrically conductive optically transparent coating on the “window” of the optical cavity. This technique provides uniform temperature control across the entire field, thus eliminating the temperature gradient that occurs with other designs. This technique also enables the FCS2 to recover from perfusion induced temperature fluctuations in just seconds.

Temperature Control:
The FCS2 was designed to maintain accurate thermal control and allow high-volume laminar flow perfusion. Both of these functions are incorporated into our patented Microaqueduct Slide (see drawing below). The surface of the slide, opposite the specimen side, is coated with an electrically conductive transparent thin film of Indium-Tin Oxide (ITO) and two electrical contacts (busbars). When the FCS2 is completely assembled two electrical contacts, (not shown in drawing), which are contained in the electrical enclosure rest on the busbars. A temperature controller is used to pass a regulated current flow through the ITO Coating. This causes the surface of the slide to heat. The heat is transferred through the perfusable media to the cell surface on the coverslip thereby providing first surface thermal control. The self-locking base of the chamber is also temperature regulated to provide peripheral heat as well.

Thermographic Images:

vislightfcs2
fcs237deg


The thermograph above demonstrates the uniform temperature distribution of an FCS3. Notice that the coverslip temperature is so uniform that its location, in infrared, is indistinguishable from the base of the chamber. This demonstrates the effectiveness of the ITO heated Microaqueduct Slide. It is capable of re-equilibrating cell temperature within seconds of perfusion and eliminates the typical thermal gradient that occurs with peripheral heating.

Microaqueduct Perfusion:
A fluid pathway is formed by separating the Microaqueduct Slide from the coverslip containing cells with a single silicone gasket. This gasket can be any thickness from 50 micron to 1mm and any lateral geometry you choose or create. This arrangement allows the user to define the flow characteristics. Therefore, you are not limited by the geometry of the optical cavity. Instead you select or create it! Fluid access to this flow channel is made through two 14-gauge needle stock tubes protruding from the sides of the chamber top. These tubes provide fluid connection to two perfusion holes in the Microaqueduct Slide that interface two “T” shaped grooves cut into the inner surface of the Microaqueduct Slide. The “T” groove allows the media to seek the path of least resistance and become nearly laminar before flowing across the cells. This technique eliminates the need for the metal perfusion ring and additional gaskets, which are the limiting factors, required by most conventional chambers. (see drawing below)

microaquflo microaqfcs2


Isometric View of Optical Cavity
Microaqueduct Slide is transparent glass but rendered as a solid to improve contrast to illustrate function. Microaqueduct design enables proper Koehler illumination with high-numeric aperture optics for both transmitted and reflected modes of microscopy. Suitable for static or high rate of flow procedures where a rapid exchange of media is required with low cell surface shear Cell temperature can be controlled from ambient to 50 degrees C +/- 0.2 degrees C without the need of an air curtain. Temperature is controlled uniformly across entire field with media equilibrating as it enters the chamber. (see drawing above)


FCS2 Chamber Features

  • Closed system so that bicarbonate CO2 or organic buffers can be employed
  • Compatible with 1/16″ tubing for perfusion (C-Flex, Tygon, etc.)
  • Easily assembled with ordinary skill (no tools required)
  • Stand-alone temperature controller with an alarm circuit to safeguard your cells
  • Near laminar flow


Exploded View of FCS2 Chamber
fcs2_explode

  1. Electrical Enclosure
    Can also be detached to sterilize the perfusion tubes
    Temperature sensor
    Heater contacts
  2. Upper Half (Top)
    Contains the perfusion tubes
  3. Perfusion Tubes (14 gauge) (CO2 Perfusion Drawing)
  4. Upper Gasket
  5. Microaqueduct Slide
    An optical surface which integrates perfusion and temperature control
    High-volume laminar flow
    Koehler Illumination
    Electronically conductive coating for temperature control
  6. Singular lower gasket
    This gasket can have any internal geometry you desire
    Standard thicknesses from .1mm to 1mm
    Allows you to define the volume and flow characteristics of the chamber
  7. 40mm coverslip
    Surface where your cells are grown
  8. Self locking base
    Designed to assure parallel uniform closure, eliminate leaks, & broken coverslips
    Temperature controlled
    Dovetail mounted to scope for stability
    No tools for assembly

Items 5,6 & 7 are the optical cavity, the gasket (#6) can be changed to any one of the gaskets below or a custom gasket can be made to make any flow geometry, or media volume you want. The coverslip comes in a standard 0.17mm, #1.5 thickness but is also available in 0.5mm, #5 coverslip.


Singular lower gasket
By simply changing this one gasket you can change the volume of the chamber. This gasket can have any internal geometry you desire and can be any thickness from 0.1mm to 1mm. The drawing below shows the standard shapes of the gaskets that we include with every FCS2. We also include solid gaskets for you to custom fit to your application. Once you have found the shape that works best for your experiment, you can contact us to have a die made to those specifications. Examples of standard gasket outlines (below)

stdgasketset

Standard Gasket Set includes:
(3) 0.1mm Thick 30mm Round
(2) 0.1mm Thick 14 x 24 Rectangle
(3) 0.25mm Thick 30 mm Round
(2) 0.25mm Thick 14 x 24 Rectangle
(1) 0.25mm Thick Blank
(3) 0.5mm Thick 30mm Round
(2) 0.5mm Thick 14 x 24 Rectangle (2) 0.5mm Thick Blank
(5) 0.75mm Thick 30mm Round w/holes
(2) 0.75mm Thick 14 x 24 Rectangle
(1) 0.75mm Thick Blank
(2) 1.0mm Thick 30mm Round
(1) 1.0mm Thick 14 x 24 Rectangle
(1) 1.0mm Thick Blank

To order a specific geometry please see the price list for part numbers and prices. Gaskets are sold 5 gaskets per pack.
In addition to the standard gasket set that comes with the FCS2 Starter Kit, shown above, there are a variety of gasket geometries, and thicknesses ranging from 0.1mm to 1mm available. Custom thicknesses and materials available upon request. See the additional gaskets shapes chart below.
FCS_Gaskets
Custom shapes are available. Simply contact Bioptechs to make arrangements for their production. All dimensions below are in millimeters. Please specify the die number that is located on the gasket drawing below and the thickness of the gasket when ordering.

Perfusion Diagram for FCS2


To assist you and Bioptechs in selecting the most appropriate system to meet your needs, please review and fill out the following questionnaire.
Micro-Environmental System Profile Questions

 

FCS2 Starter Set includes:

  • FCS2 Chamber
  • Controller
  • 5 Micro aqueduct Slides
  • 100 #1.5 – 40mm German Desag263 Coverslips
  • 30 piece Gasket Set

Standard FCS2 Chamber Specifications:

Physical Size 75mm OD 13mm high
Coverslip No. 1.5 thick x 40mm Diameter
Imaging Aperture 22mm
Maximum Volume 706mm^3
Minimum Volume 4mm^3
Maximum Volume Exchange Rate 1/sec
Minimum Fluid Aperture 0.6mm^2
Separation between optical surfaces 50 – 1000 microns
External port ID 1.6mm
Temperature Stability +/- 0.2 degrees C

Standard FCS2 Top:

Tube Tube Length (mm) Tube Inside Diameter (mm) Dead Volume (µl)
A 29 1.57 56.14
B 10 0.88 6.08
Total Dead Volume 62.22

 

FCS2 System
FCS2 Starter Set (includes:) 060319-2 $5,970.80
FCS2 Chamber, Chamber Controller    
5 Microaqueduct Slides, 40mm Coverslip (100 pk),    
and Gasket Set (30/set)
FCS2 Chamber 060319-2-03 $2,462.96
FCS2/3 Chamber Controller 060319-2-0303 $2,985.40
Controller Power Supply 19-01 $37.95
No-Heat Flow Cell (FCS2) 03060319-2-NH $2,686.86
Includes: Non-heated FCS2 Chamber, (5) Non-heating Microaqueduct Slides, (50) #1.5 – 40mm Coverslips, (30 piece) gasket set
FCS2 Stage Adapters
Leica DMIRB Triple Plate Adapter 060319-2-12 $820.99
Nikon, Olympus, Deltavision Round Adapter 060319-2-1415 $559.77
Nikon Round Adapter (Nikon Only) 060319-2-15 $559.77
Marzhauser Slim Stage 100×80 (160mmx116mm) 060319-2-1219 $1,119.53
Marzhauser, Zeiss (K), ASI, Ludl & Prior Adapter 060319-2-2611 $970.26
SBS / 96 Well Plate Adapter 060319-2-2003 $948.75
Zeiss (M) & Leica Single Plate Adapter 060319-2-2613 $746.35
Mad City Labs Stage Adapter 060319-2-13319 $1,119.53
FCS2 & FCS3 Supplies
Microaqueduct Slides (5/pk) 130119-5 $522.45
40mm Coverslips (200 pk) 40-1313-0319 $241.61
40mm Coverslips (500 pk) 40-1313-03192 $522.45
40mmCoverslips (1000 pk) 40-1313-03193 $970.26
FCS2/3 Gasket Set (30/set) 060319-2-0719 $223.91
30mm Round w/holes 0.75mm Thick – 5/pack 1907-08-750 $37.32
Blank Gasket .1mm Thick – 5/pack 1907-100 $37.32
Blank Gasket .25mm Thick – 5/pack 1907-250 $37.32
Blank Gasket .5mm Thick – 5/pack 1907-500 $37.32
Blank Gasket .75mm Thick – 5/pack 1907-750 $37.32
Blank Gasket 1mm Thick – 5/pack 1907-1000 $37.32
14 x 24 Rectangle Gasket .1mm thick – 5/pack 1907-1422-100 $37.32
14 x 24 Rectangle Gasket .25mm thick – 5/pack 1907-1422-250 $37.32
14 x 24 Rectangle Gasket .5mm thick – 5/pack 1907-1422-500 $37.32
14 x 24 Rectangle Gasket .75mm thick – 5/pack 1907-1422-750 $37.32
14 x 24 Rectangle Gasket 1mm thick – 5/pack 1907-1422-1000 $37.32
30mm Round Gasket .1mm thick – 5/pack 1907-18-100 $37.32
30mm Round Gasket .25mm thick – 5/pack 1907-18-250 $37.32
30mm Round Gasket .5mm thick – 5/pack 1907-18-500 $37.32
30mm Round Gasket .75mm thick – 5/pack 1907-18-750 $37.32
30mm Round Gasket 1mm thick – 5/pack 1907-18-1000 $37.32
Custom Gasket (5pk) Die # on Guide $37.32
FCS2 Accessories
FCS2 Cooling Adapter 060319-2-0301 $597.08
Open Mode Adapter 060319-2-1513 $260.54
FCS2 Low Dead Volume Top 060319-2-1242 $738.89
Cooled FCS2 (CFCS2) 03060319-2 $3731.75
Perfusion
FCS2 Micro-Perfusion Pump (Low-Flow) 060319131616 $1,195.00
FCS2 Micro-Perfusion Pump (Ultra Low-Flow) 0603191310683 $1,350.00
FCS2 Micro-Perfusion Pump (High-Flow) 0603191372066 $1,350.00
FCS2 Single Tube Pump Set (4/pk) C-Flex .062″ ID 060319192016 $137.50
FCS2 Single Tube Pump Set (4/pk) Silicon .062″ ID 060319192016S $137.50
FCS2 Single Tube Pump Set (4/pk) C-Flex .031″ID 060319031C31 $137.50
FCS2 Single Tube Pump Set (4/pk) C-Flex .020″ ID 060319020C20 $137.50
FCS2 Single Tube Pump Set (4/pk) Silicone .015″ID 060319015S15 $137.50
C-Flex Tubing (50ft) 202050 $62.50
1/16″ Perfusion Adapter Set 162003-1 $28.75
Micro-Perfusion Pump Rod Mounting Clamp 16181303 $37.95
CO2 Metering Pump 03152-16 $600.00
Oasis Heating & Cooling System (2 to 45 C) 15-160 $3,125.00
Perfusion / Temperature Controller (USB Interface) 13161603-13 $3,680.00

FCS2-3Comparison